ESAT6 (AA 21-95) Antikörper

Produktnummer ABIN1715132

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-ESAT6 Antikörper wird verwendet zum Nachweis von ESAT6 in Proben von Bakterien, Maus und Mykobakterien. Er wurde validiert für WB, ELISA, ICC, IF (cc), IF (p), IHC (fro) und IHC (p).

Kurzübersicht für ESAT6 (AA 21-95) Antikörper (ABIN1715132)

Target: ESAT6

Reaktivität: Bakterien, Maus, Mykobakterien

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 21-95

Kreuzreaktivität: Bakterien, Maus

Kreuzreaktivität (Details): Mycobacterium

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis ESAT6

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen

Koyama, Ito, Hasegawa, Eriguchi, Inaba, Ushigusa, Sugiura: "Exosomes derived from tumor cells genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy." in: Biotechnology letters, Vol. 38, Issue 11, pp. 1857-1866, (2016) (PubMed).

Antigendetails

Target: ESAT6

Hintergrund:

Synonyms: 6 kDa early secretory antigenic target, 6 kDa early secretory antigenic target ESXA, ESAT 6, esaT6, ESXA, ESXA_MYCTU.

Background: Mycobacterium tuberculosis is a slow-growing obligate aerobic bacillus that causes most cases of tuberculosis (TB). It is a small, rod-like microbe that can withstand weak disinfectants and survive in a dry state for weeks, but can only grow within a host organism. M. tuberculosis has a thick waxy cell wall that is responsible for the typical caseous granuloma formation in tuberculosis. ESAT6 is a protein secreted by M. tuberculosis that induces a strong immune response in infected organisms, making it a strong candidate for the development of a vaccine against tuberculosis. ESAT6 may also be useful in the detection of the disease. Deletion of the ESAT6 gene leads to the loss of virulence of M. tuberculosis.