GFI1 Antikörper (AA 301-400)

Produktnummer ABIN1714623

Dieses Anti-GFI1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GFI1 in WB, ELISA, IHC (p), ICC, IF (cc), IF (p) und IHC (fro). Geeignet für Ratte, Maus und Xenopus laevis.

Kurzübersicht für GFI1 Antikörper (AA 301-400) (ABIN1714623)

Target: GFI1 (Growth Factor Independent 1 (GFI1))

Reaktivität: Ratte, Maus, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-GFI1 Antikörper

Bindungsspezifität: AA 301-400

Kreuzreaktivität: Maus, Ratte, Xenopus laevis

Homologie: Human,Dog,Sheep,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFI1/ZNF163

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu GFI1

Target: GFI1 (Growth Factor Independent 1 (GFI1))

Andere Bezeichnung: GFI1/ZNF163

Hintergrund:

Synonyms: GFI 1, GFI1, GFI-1, GFI1_HUMAN, Growth factor independence 1, growth factor independent 1, Growth factor independent protein 1, SCN2, Zinc finger protein 163, Zinc finger protein Gfi 1, Zinc finger protein Gfi-1, Zinc finger protein Gfi1, ZNF 163, ZNF163.

Background: Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages. Gfi-1B, a related protein, is a transcriptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the Socs 1 and 3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.3, respectively.

Gen-ID: 2672