ETF1 Antikörper (AA 341-437)

Produktnummer ABIN1714530

Produktdetails anti-ETF1 Antikörper

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Bindungsspezifität: AA 341-437

Reaktivität: Xenopus laevis, Kugelfisch

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ETF1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Kugelfisch, Xenopus laevis

Homologie: Human,Mouse,Rat,Dog,Cow,Horse,Zebrafish,Arabidopsis Thaliana

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eRF1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Andere Bezeichnung: eRF1 (ETF1 Produkte)

Synonyme: D5S1995 antikoerper, ERF antikoerper, ERF1 antikoerper, RF1 antikoerper, SUP45L1 antikoerper, TB3-1 antikoerper, AI463371 antikoerper, D6Ertd109e antikoerper, eRF1 antikoerper, zeh0057 antikoerper, hm:zeh0057 antikoerper, wu:fa19e07 antikoerper, wu:fi31f01 antikoerper, cl1 antikoerper, RRF1 antikoerper, DDBDRAFT_0188023 antikoerper, DDBDRAFT_0191343 antikoerper, DDB_0188023 antikoerper, DDB_0191343 antikoerper, CG5605 antikoerper, Dm0184 antikoerper, Dmel\\CG5605 antikoerper, Group J antikoerper, group J antikoerper, l(3)00103 antikoerper, l(3)05637 antikoerper, l(3)77ABb antikoerper, l(3)neo28 antikoerper, Eukaryotic release factor 1 antikoerper, eukaryotic translation termination factor 1 antikoerper, eukaryotic translation termination factor 1b antikoerper, eukaryotic translation termination factor 1 S homeolog antikoerper, eukaryotic peptide chain release factor subunit 1 antikoerper, eukaryotic petide chain release factor subunit 1 antikoerper, eukaryotic release factor 1 antikoerper, Eukaryotic peptide chain release factor subunit 1 antikoerper, ETF1 antikoerper, Etf1 antikoerper, etf1b antikoerper, etf1 antikoerper, etf1.S antikoerper, Tb11.22.0012 antikoerper, THAPSDRAFT_22240 antikoerper, TTHERM_00292170 antikoerper, LOC692937 antikoerper, erf1 antikoerper, eRF1 antikoerper, erfa-1 antikoerper

Hintergrund:

Synonyms: Cl1 protein, D5S1995, ERF, eRF1, ERF1_HUMAN, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Eukaryotic translation termination factor 1, MGC111066, Polypeptide chain release factor 1, Protein Cl1, RF1, Sup45 yeast omnipotent suppressor 45 homolog like 1, SUP45L1, TB3 1, TB3-1.

Background: Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the GTP state,????????? it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the GDP state,????????? it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.

Gen-ID: 2107