RUNX3 Antikörper (AA 31-130)

Produktnummer ABIN1714069

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch RUNX3 in WB, ELISA, IF (p), FACS, IF (cc), IHC (p), ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für RUNX3 Antikörper (AA 31-130) (ABIN1714069)

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RUNX3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RUNX3 Antikörper

Bindungsspezifität: AA 31-130

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Runx3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #029621 (Immunofluorescence)

by: CaresBio Laboratory

No.: #029621

Datum: 08.03.2014

Chargennummer: 131016

Validierte Anwendung: Immunofluorescence

Positivkontrolle: A431 cells

Negativkontrolle: HEK293 cells

Bewertung: Strong signal was detected in the positive control, and no signal was detected in the negative control.

Validierungsbilder

Figure 1: A431 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).

Figure 2: HEK293 cells stained with anti-RUNX3 (green) and counterstained with DAPI (blue).

Figure 3: A431 cells stained with isotype control (green) and counterstained with DAPI (blue).

Figure 4: A431 cells stained with secondary antibody only (green) and counterstained with DAPI (blue).

Protokoll

Primärantikörper:

• Antigen: Runt-Related Transcription Factor 3 (RUNX3)
• Catalog number: ABIN1714069
• Lot number: 131016

Sekundärantikörper:

• Antibody: Donkey Anti-Rabbit secondary antibody IgG (H+L) AlexaFluor 488

Full Protocol:

• Antibody staining:
• A431 and Hek-293 cell lines were grown directly on coverslips and fixed with 4% formaldehyde in PBS for 15 min at room temperature (RT).
• Fixed cells were rinsed three times in PBS for 5 min each at RT.
• Cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT.
• Cells were incubated with primary antibody diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.
• Isotype Control Staining:
  
• All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 to block unspecific binding of the antibodies for 60 min at RT. - Cells were incubated with 10% normal rabbit serum overnight at 4°C.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Cells were incubated with donkey anti rabbit FITC conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.
• Secondary Antibody Only Control Staining:
  
• All the steps are done same as previously described until cells were blocked in 1X PBS/1% BSA/0.3% Triton™ X-100 overnight at 4°C. - Cells were incubated with donkey anti rabbit Alexa488 conjugated secondary antibody for 60 min in dark at RT.
• Cells were rinsed three times in PBS for 5 min each at RT.
• Coverslips were mounted on slides with ProLong® Gold Antifade Reagent with DAPI.
• Stained cells were imaged with an Olympus IX73 microscope.

Anmerkungen:

• No discrepancies reported.
• Runx3 antibody is recommended for IF on A431 cells according to this protocol.

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu RUNX3

Target: RUNX3 (Runt-Related Transcription Factor 3 (RUNX3))

Andere Bezeichnung: Runx3

Hintergrund:

Synonyms: RUNX 3, RUNX-3, Runt-related transcription factor 3, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, OncogeneAML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, Core-binding factor, alpha 3 subunit, CBF-alpha 3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, PEBP2-alpha C, PEA2-alpha C, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit.

Background: RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus.

Gen-ID: 864