DPPA3 Antikörper (AA 101-159)

Produktnummer ABIN1713577

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DPPA3 in WB, ELISA, ICC, IHC (p), IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus und Schwein und wurde in 1 Publikation erwähnt.

Kurzübersicht für DPPA3 Antikörper (AA 101-159) (ABIN1713577)

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Reaktivität: Maus, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPPA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-DPPA3 Antikörper

Bindungsspezifität: AA 101-159

Kreuzreaktivität: Maus, Schwein

Homologie: Human

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Stella/DPPA3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-DPPA3 Antikörper (ABIN1713577)

Wasielak, Więsak, Bogacka, Jalali, Bogacki: "Zygote arrest 1, nucleoplasmin 2, and developmentally associated protein 3 mRNA profiles throughout porcine embryo development in vitro." in: Theriogenology, Vol. 86, Issue 9, pp. 2254-2262, (2016) (PubMed).

Details zu DPPA3

Target: DPPA3 (Developmental Pluripotency Associated 3 (DPPA3))

Andere Bezeichnung: Stella/DPPA3

Hintergrund:

Synonyms: STELLA, Developmental pluripotency-associated protein 3, Stella-related protein, DPPA3, STELLAR

Background: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity).

Gen-ID: 359787

UniProt: Q6W0C5