SP1 Antikörper (pThr453) (HRP)

Produktnummer ABIN1711481

Der Kaninchen Polyklonal Anti-SP1-Antikörper wurde für WB, ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, SP1 in Proben von Maus zu detektieren.

Kurzübersicht für SP1 Antikörper (pThr453) (HRP) (ABIN1711481)

Target: SP1 (Sp1 Transcription Factor (SP1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SP1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SP1 Antikörper (HRP)

Bindungsspezifität: pThr453

Kreuzreaktivität: Maus

Homologie: Human,Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human TSFP1 around the phosphorylation site of Thr453

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu SP1

Target: SP1 (Sp1 Transcription Factor (SP1))

Andere Bezeichnung: SP1

Hintergrund:

Synonyms: SP1 Thr453, SP1 T453, p-SP1 phospho T453, p-TSFP1 phospho T453, Sp1 transcription factor isoform a, TSFP1, TSFP 1, Specicity protein 1, Transcription factor Sp1, SP 1, SP1, Sp1 transcription factor, SP1_HUMAN.

Background: Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21-bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A) (7), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.

Gen-ID: 6667

Pathways: Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction