DCLRE1C Antikörper (AA 301-400) (Cy5)

Produktnummer ABIN1704476

Der Kaninchen Polyklonal Anti-DCLRE1C-Antikörper wurde für WB, IF (cc) und IF (p) validiert. Er ist geeignet, DCLRE1C in Proben von Ratte zu detektieren.

Kurzübersicht für DCLRE1C Antikörper (AA 301-400) (Cy5) (ABIN1704476)

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCLRE1C Antikörper ist konjugiert mit Cy5

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-DCLRE1C Antikörper (Cy5)

Bindungsspezifität: AA 301-400

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Cow,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DCLREC1C/Artemis

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Andere Bezeichnung: DCLREC1C/Artemis

Hintergrund:

Synonyms: SCIDA, SNM1C, A-SCID, RS-SCID, DCLREC1C, Protein artemis, DNA cross-link repair 1C protein, Protein A-SCID, SNM1 homolog C, hSNM1C, SNM1-like protein, DCLRE1C, ARTEMIS, ASCID

Background: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Gen-ID: 64421

UniProt: Q96SD1

Pathways: DNA Reparatur