POLI Antikörper (AA 51-150) (AbBy Fluor® 488)

Produktnummer ABIN1695485

Der Kaninchen Polyklonal Anti-POLI-Antikörper wurde für IF (cc) und IF (p) validiert. Er ist geeignet, POLI in Proben von Ratte zu detektieren.

Kurzübersicht für POLI Antikörper (AA 51-150) (AbBy Fluor® 488) (ABIN1695485)

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLI Antikörper ist konjugiert mit AbBy Fluor® 488

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-POLI Antikörper (AbBy Fluor® 488)

Bindungsspezifität: AA 51-150

Kreuzreaktivität: Ratte

Homologie: Human,Mouse,Dog

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA Polymerase iota

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Andere Bezeichnung: DNA Polymerase iota

Hintergrund:

Synonyms: DNA polymerase iota, Eta 2, Eta2, POLI, POLI_HUMAN, Polymerase DNA directed iota, RAD 30B, RAD30 homolog B, RAD30B, RAD3OB.

Background: DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol , which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.

Gen-ID: 11201

Pathways: DNA Reparatur