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GIT1 Antikörper (AA 375-770)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GIT1 in WB, IP, IF und ICC. Er zeigt eine Reaktivität gegenüber Ratte.
Produktnummer ABIN1686622

Kurzübersicht für GIT1 Antikörper (AA 375-770) (ABIN1686622)

Target

Alle GIT1 Antikörper anzeigen
GIT1

Reaktivität

  • 79
  • 55
  • 42
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 106
  • 21
Maus

Klonalität

  • 97
  • 30
Monoklonal

Konjugat

  • 45
  • 8
  • 8
  • 7
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GIT1 Antikörper ist unkonjugiert

Applikation

  • 94
  • 39
  • 39
  • 35
  • 29
  • 19
  • 18
  • 18
  • 16
  • 9
  • 8
  • 4
  • 2
  • 2
  • 2
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon

S39B-8
  • Bindungsspezifität

    • 16
    • 15
    • 15
    • 15
    • 9
    • 8
    • 7
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 375-770

    Spezifität

    Detects ~90 kDa. Does not cross-react with GIT2.

    Kreuzreaktivität

    Human, Maus, Ratte

    Aufreinigung

    Protein G Purified

    Immunogen

    Fusion protein amino acids 375-770 (C-terminus) of rat GIT1

    Isotyp

    IgG1
  • Applikationshinweise

    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    1 μg/ml of ABIN1686622 was sufficient for detection of GIT1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    -20°C
  • Target

    GIT1

    Andere Bezeichnung

    GIT1

    Hintergrund

    G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).

    Gen-ID

    83709

    NCBI Accession

    NP_114002

    UniProt

    Q9Z272
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