Huntingtin Antikörper (N-Term)

Produktnummer ABIN1607890

Produktdetails anti-Huntingtin Antikörper

Target: Huntingtin (HTT)

Bindungsspezifität: N-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Huntingtin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Kreuzreaktivität (Details): Cross reactivity with HTT from other sources has not been determined.

Reinheit: Anti-Huntington was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HTT. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, and Rat based on 100 % sequence homology.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington.
Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise: Anti-Huntington antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~350 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 0.1 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: Expiration date is six (6) months from date of opening.

Details zu Huntingtin

Target: Huntingtin (HTT)

Andere Bezeichnung: Huntington (HTT Produkte)

Synonyme: HTT antikoerper, hd antikoerper, ZHD antikoerper, CG9995 antikoerper, Dmel\\CG9995 antikoerper, HD antikoerper, Hsap\\HD antikoerper, Htt antikoerper, dHtt antikoerper, dhtt antikoerper, SLC6A4 antikoerper, huntington antikoerper, it15 antikoerper, htt antikoerper, IT15 antikoerper, AI256365 antikoerper, C430023I11Rik antikoerper, Hd antikoerper, Hdh antikoerper, huntingtin antikoerper, HTT antikoerper, htt antikoerper, LOC373520 antikoerper, Htt antikoerper

Hintergrund: Huntington (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Anti-Huntingtin antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research. Synonyms: HD protein, HTT, huntingtin, huntingtin (Huntington disease), IT15HD, Huntington disease protein

Gen-ID: 3064

NCBI Accession: NP_002102

UniProt: P42858

Pathways: PI3K-Akt Signalweg, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport