CX3CL1 Antikörper

Produktnummer ABIN1607860

Produktdetails anti-CX3CL1 Antikörper

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CX3CL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity with Fractalkine from other sources has not been determined.

Reinheit: Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100 % homology with the immunizing sequence.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Fractalkine purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Western Blot Dilution: 1:1000-1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Andere Bezeichnung: Fractalkine (CX3CL1 Produkte)

Synonyme: ABCD3 antikoerper, CX3CL1 antikoerper, DKFZp459K117 antikoerper, Cx3c antikoerper, Scyd1 antikoerper, ABCD-3 antikoerper, C3Xkine antikoerper, CXC3 antikoerper, CXC3C antikoerper, NTN antikoerper, NTT antikoerper, SCYD1 antikoerper, fractalkine antikoerper, neurotactin antikoerper, AB030188 antikoerper, AI848747 antikoerper, CX3C antikoerper, Cxc3 antikoerper, D8Bwg0439e antikoerper, ATP binding cassette subfamily D member 3 antikoerper, C-X3-C motif chemokine ligand 1 antikoerper, chemokine (C-X3-C motif) ligand 1 antikoerper, ABCD3 antikoerper, CX3CL1 antikoerper, Cx3cl1 antikoerper, Abcd3 antikoerper

Hintergrund: Fractalkine (CX3CL1) is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. It is found predominantly in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium by binding to CX3CR1. Recombinant human Fractalkine is a non-glycosylated protein with a molecular weight of 8.6 kDa. Anti-Fractalkine antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: CX3CL1, Fractalkine, Chemokine (C-X3-C motif) ligand 1, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1, A-152E5.2

Gen-ID: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane