Albumin Antikörper (HRP)

Produktnummer ABIN1607852

Hersteller Produkt- Nr.: 201-4333-0100

Produktdetails anti-Albumin Antikörper (HRP)

Target: Albumin (ALB)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Albumin Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 201-4333-0100

Hersteller: Rockland

Verwendungszweck: Bovine Serum Albumin Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].

Produktmerkmale: Synonyms: rabbit anti-bovine albumin peroxidase conjugation, rabbit anti-bovine albumin HRP conjugation, rabbit anti-BSA HRP conjugated antibody, rabbit anti-bovine serum albumin peroxidase conjugated antibody, ALB antibody, Albumin antibody, HRP conjugated Anti-Bovine serum albumin antibody

Aufreinigung: Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Albumin (Bovine Serum)

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:250 - 1:1,000

Application Note: Anti-BOVINE ALBUMIN Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. It is also used as a nutrient in cell and microbial culture. Specific conditions should be optimized by user.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: ALB (ALB Produkte)

Hintergrund: Background: Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Anti-Bovine albumin antibody is ideal for investigators involved in serum protein component research.

Gen-ID: 280717

UniProt: P02769

Pathways: Lipid Metabolism