RNASE1 Antikörper (Biotin)

Produktnummer ABIN1607821

Hersteller Produkt- Nr.: 200-4688-0100

Produktdetails anti-RNASE1 Antikörper (Biotin)

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNASE1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Hersteller Produkt- Nr.: 200-4688-0100

Hersteller: Rockland

Verwendungszweck: Ribonuclease A Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Ribonuclease A Antibody biotin Conjugation, biotin Conjugated rabbit anti-Ribonuclease A Antibody, Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

Aufreinigung: Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ribonuclease A [Bovine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Ribonuclease A Biotin Conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:13,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:150,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu RNASE1

Target: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Andere Bezeichnung: RNASE1 (RNASE1 Produkte)

Hintergrund: Background: Anti-Ribonuclease A recognizes ribonuclease, an endoribonuclease that catalyzes the cleavage of the phosphodiester bond between 5'-ribose of a nucleotide and the phosphate group of a 3'-ribose of a pyrimidine nucleotide. Able to survive acidic and near boiling conditions, RNase A is highly stable. RNase A and its homologs have been demonstrated to have cytotoxic and cytostatic effects.

Gen-ID: 282340

UniProt: P61823