DNASE1 Antikörper (Biotin)

Produktnummer ABIN1607795

Hersteller Produkt- Nr.: 200-4661-0100

Dieses Anti-DNASE1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DNASE1 in WB, ELISA und IP. Geeignet für Rind (Kuh).

Kurzübersicht für DNASE1 Antikörper (Biotin) (ABIN1607795)

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNASE1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-DNASE1 Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-4661-0100

Hersteller: Rockland

Verwendungszweck: Deoxyribonuclease I Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].

Produktmerkmale: Synonyms: rabbit anti-Deoxyribonuclease I Antibody biotin Conjugation, biotin conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

Aufreinigung: Anti-Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Deoxyribonuclease I [Bovine Pancreas]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Deoxyribonuclease I Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Deoxyribonuclease I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Andere Bezeichnung: DNASE1, DNL1

Hintergrund: Background: Anti-Deoxyribonuclease I antibody is primarily involved in cell death by apoptosis, though it performs other various functions. Deoxyribonuclease I binds specifically to G-actin and blocks actin polymerization. Belonging to the DNase I family, Anti-Deoxyribonuclease antibody is ideal for researchers interested in Metabolism, Epigenetics and Nuclear Signaling, and Cell Biology.

Gen-ID: 282217

NCBI Accession: NP_776959

UniProt: P00639