GLUD1 Antikörper (Biotin)

Produktnummer ABIN1607789

Produktdetails anti-GLUD1 Antikörper (Biotin)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Glutamate Dehydrogenase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Glutamate Dehydrogenase [Bovine Liver]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Glutamate Dehydrogenase has been assayed against 1.0 µg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: Glutamate Dehydrogenase (GLUD1 Produkte)

Synonyme: GDH antikoerper, gdh1 antikoerper, GDH 1 antikoerper, glud1 antikoerper, GLUD1 antikoerper, cb719 antikoerper, wu:fb16e02 antikoerper, wu:fb58f12 antikoerper, wu:fe37f03 antikoerper, wu:fj43f02 antikoerper, zgc:192851 antikoerper, zgc:55630 antikoerper, GDH1 antikoerper, GLUD antikoerper, AI118167 antikoerper, Gdh-X antikoerper, Glud antikoerper, Gludl antikoerper, Ac2-281 antikoerper, Gdh1 antikoerper, Gludeha antikoerper, MRG-2 antikoerper, GLUTAMATE DECARBOXYLASE 1 antikoerper, GLUTAMATE DEHYDROGENASE 1 antikoerper, MRG7.13 antikoerper, MRG7_13 antikoerper, glutamate dehydrogenase 1 antikoerper, C2H1orf130 antikoerper, legdh1 antikoerper, cb622 antikoerper, wu:fc33g09 antikoerper, wu:fc66a10 antikoerper, zgc:77186 antikoerper, glutamate dehydrogenase 1 antikoerper, glutamate dehydrogenase 1 S homeolog antikoerper, glutamate dehydrogenase 1, mitochondrial antikoerper, glutamate dehydrogenase 1b antikoerper, glutamate dehydrogenase antikoerper, non-compact myelin associated protein antikoerper, glutamate dehydrogenase 1a antikoerper, GLUD1 antikoerper, GDH1 antikoerper, glud1 antikoerper, glud1.S antikoerper, LOC693461 antikoerper, glud1b antikoerper, Glud1 antikoerper, HACJB3_RS00320 antikoerper, LOC100587725 antikoerper, NCMAP antikoerper, gdh1 antikoerper, glud1a antikoerper

Hintergrund: Anti-glutamate dehydrogenase antibody recognizes the glutamate dehydrogenase protein. Glutamate dehydrogenase is an enzyme that is present in the mitochondria of eukaryotes. Glutamate dehydogenase plays a role in urea synthesis by converting glutamate to α-ketoglutarate, and vice versa. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate. Glutamate dehydrogenase also has a very high affinity for ammonia. Therefore toxic levels of ammonia would have to be present in the body for the reverse reaction to proceed. In bacteria, the ammonia is assimilated to amino acids via glutamate and amidotransferases. In plants, the enzyme can work in either direction depending on environment and stress. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. Anti-glutamate dehydrogenase antibody is suitable for researchers in Cell Biology and Immunology. Synonyms: Glutamate dehydrogenase 1, mitochondrial GDH 1 EC=1.4.1.3

Gen-ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt