GLUD1 Antikörper (Biotin)

Produktnummer ABIN1607789

Hersteller Produkt- Nr.: 200-4658-0100

Dieses Anti-GLUD1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GLUD1 in WB, IP und DB. Geeignet für Rind (Kuh).

Kurzübersicht für GLUD1 Antikörper (Biotin) (ABIN1607789)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Produktdetails anti-GLUD1 Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-4658-0100

Hersteller: Rockland

Verwendungszweck: Anti-Glutamate Dehydrogenase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Produktmerkmale: Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1

Aufreinigung: Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Glutamate Dehydrogenase [Bovine Liver]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Glutamate Dehydrogenase Biotin Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: GLUD1

Hintergrund: Background: Anti-glutamate dehydrogenase antibody recognizes the glutamate dehydrogenase protein. Glutamate dehydrogenase is an enzyme that is present in the mitochondria of eukaryotes. Glutamate dehydrogenase plays a role in urea synthesis by converting glutamate to α-ketoglutarate, and vice versa. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals as glutamate dehydrogenase equilibrium favors the production of ammonia and α-ketoglutarate. Glutamate dehydrogenase also has a very high affinity for ammonia. Therefore toxic levels of ammonia would have to be present in the body for the reverse reaction to proceed. In bacteria, the ammonia is assimilated to amino acids via glutamate and aminotransferases. In plants, the enzyme can work in either direction depending on environment and stress. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. Anti-glutamate dehydrogenase antibody is suitable for researchers in Cell Biology and Immunology.

Gen-ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt