Dieser beta-2 Microglobulin Antikörper ist konjugiert mit Biotin
Applikation
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Kreuzreaktivität (Details)
Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
Reinheit
Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine. Immunogen Type: Native Protein
Anti-beta-2-Microglobulin antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below. ELISA Dilution: 1:2.000 - 1:10.000 Immunohistochemistry Dilution: 1:500 - 1:3.000 Western Blot Dilution: 1:1.000 - 1:5.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.
Keefe, Hwang, Huan, Mendelson, Yao, Courchesne, Saleh, Madhur, Levy: "Evidence for a Causal Role of the SH2B3-β2M Axis in Blood Pressure Regulation." in: Hypertension (Dallas, Tex. : 1979), Vol. 73, Issue 2, pp. 497-503, (2019) (PubMed).
Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research. Synonyms: B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody