ATF6 Antikörper (AA 437-449)

Produktnummer ABIN1590087

Dieses Anti-ATF6-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ATF6 in WB, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für ATF6 Antikörper (AA 437-449) (ABIN1590087)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ATF6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ATF6 Antikörper

Bindungsspezifität: AA 437-449

Verwendungszweck: ATF6 (aa437-449)

Sequenz: NSYRYDHSVS NDK

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75-80 kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6 kDa according to NP_031374.2). Recommended concentration: 0.3-2 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Andere Bezeichnung: ATF6

Hintergrund: ATF6, activating transcription factor 6, ATF6A, cAMP-dependent transcription factor ATF-6 alpha, cyclic AMP-dependent transcription factor ATF-6 alpha

Gen-ID: 22926, 304962

NCBI Accession: NP_031374

Pathways: ER-Nucleus Signaling, Unfolded Protein Response