MAPKAP Kinase 5 Antikörper (AA 192-206)
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- Target Alle MAPKAP Kinase 5 (MAPKAPK5) Antikörper anzeigen
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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Bindungsspezifität
- AA 192-206
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Reaktivität
- Human, Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser MAPKAP Kinase 5 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Verwendungszweck
- MK5 / MAPKAPK5 (aa192-206)
- Sequenz
- PQVLEAQRRH QKEKS
- Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Schwein, Ratte
- Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Güteklasse
- Verified
- Isotyp
- IgG
- Top Product
- Discover our top product MAPKAPK5 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8 μg/mL.
Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Bioche
Peptide ELISA: antibody detection limit dilution 1:64000.
- Kommentare
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Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Minimize freezing and thawing.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- Andere Bezeichnung
- Mapkapk5 (MAPKAPK5 Produkte)
- Synonyme
- MAPKAPK5 antikoerper, MK5 antikoerper, PRAK antikoerper, MAPKAP-K5 antikoerper, MK-5 antikoerper, mitogen-activated protein kinase-activated protein kinase 5 antikoerper, MAP kinase-activated protein kinase 5 antikoerper, MAPKAPK5 antikoerper, mapkapk5 antikoerper, Mapkapk5 antikoerper
- Hintergrund
- MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase
- Gen-ID
- 8550, 17165, 498183
- NCBI Accession
- NP_003659, NP_620777
- Pathways
- MAPK Signalweg
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