MAPKAP Kinase 5 Antikörper (Internal Region)

Produktnummer ABIN1590011

Der Ziege Polyklonal anti-MAPKAP Kinase 5 Antikörper wird verwendet zum Nachweis von MAPKAP Kinase 5 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und IP.

Kurzübersicht für MAPKAP Kinase 5 Antikörper (Internal Region) (ABIN1590011)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-MAPKAP Kinase 5 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: MK5 / MAPKAPK5

Sequenz: STEALDNVLP SAQ

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney shows brushborder staining in the PCT. Recommended concentration, 4-8 μg/mL.

Western Blot: In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP, only the expected band was observed in the MK5 lane after EB11927 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemi

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Andere Bezeichnung: Mapkapk5

Hintergrund: MAPKAPK5, mitogen-activated protein kinase-activated protein kinase 5, MAPKAP-K5, MK-5, MK5, PRAK, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase

Gen-ID: 8550, 17165, 498183

NCBI Accession: NP_003659, NP_620777

Pathways: MAPK Signalweg