MAPK6 Antikörper (Internal Region)

Produktnummer ABIN1590009

Dieses Anti-MAPK6-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von MAPK6 in WB, ELISA, IHC und IP. Geeignet für Human und Maus.

Kurzübersicht für MAPK6 Antikörper (Internal Region) (ABIN1590009)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPK6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Güteklasse: Verified

Produktdetails anti-MAPK6 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ERK3 / MAPK6

Sequenz: HSPVGSPLKS IQ

Spezifität: Reported variants represent identical protein (NP_056621.4, NP_081694.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Andere Bezeichnung: Mapk6

Hintergrund: MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activate

Gen-ID: 5597, 50772, 58840

NCBI Accession: NP_002739

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C