Lamin A/C Antikörper

Produktnummer ABIN1580428

Dieses Huhn Polyklonal-Antikörper erkennt spezifisch Lamin A/C in WB, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Schwein und Säugetier.

Kurzübersicht für Lamin A/C Antikörper (ABIN1580428)

Target: Lamin A/C (LMNA)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Säugetier

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser Lamin A/C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-Lamin A/C Antikörper

Aufreinigung: IgY preparation

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:2,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 20 mg/mL

Buffer: This antibody is an IgY prep at a total protein concentration of 20mg/mL. The preparation contains 10mM sodium azide as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu Lamin A/C

Target: Lamin A/C (LMNA)

Andere Bezeichnung: Lamin A/C

Hintergrund: The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm. The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene, while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes a C-terminal segment of 98 amino acids missing from Lamin C, while Lamin C has a unique C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other. This is the case with this polyclonal, which was raised against full length recombinant human Lamin A. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of maturation promoting factor, the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging. The HGNC name for this protein is LMNA.

Pathways: Apoptose, Caspase Kaskade in der Apoptose, ER-Nucleus Signaling, Protein targeting to Nucleus