ENO1 Antikörper

Produktnummer ABIN1580416

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ENO1 in WB, IHC, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh) und Xenopus laevis.

Kurzübersicht für ENO1 Antikörper (ABIN1580416)

Target: ENO1 (Enolase 1 (ENO1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 253

Produktdetails anti-ENO1 Antikörper

Aufreinigung: affinity purified antibody

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Try at dilutions of 1:2,000-1:5,000 for immunofluorescence. For western blots try at 1:5,000-1:10,000. The NNE protein runs at about ~47 kDa on SDS-PAGE gels.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu ENO1

Target: ENO1 (Enolase 1 (ENO1))

Hintergrund: Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be refered to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as enolase 1, 2 and 3 or alternately as alpha, beta, and gamma,-enolase. Confusingly, although enolase 1 is alpha,-enolase, enolase 2 corresponds to gamma,-enolase while enolase 3 is also known as beta,-enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence, as can be seen from a sequence alignment downloadable from here. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or alpha, is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer. Enolase 2 or gamma, is also known as neuron specific enolase (NSE), and we also market antibodies directed against this protein, specifically RPCA-NSE. A switch from NNE to NSE occurs in the development of neurons. Enolase 3 or beta, is expressed primarily in muscle cells. Monoclonal antibody MCA-253 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system. The characterization of the antibody has been published in peer reviewed form and in this publication is there referred to as Enol2-53. The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1. The HGNC name for this protein is ENO1.