Podoplanin Antikörper

Produktnummer ABIN155169

Zitiert in 3+ Publikationen. Der Hamster Monoklonal anti-Podoplanin Antikörper (Klon RTD4E10) (ABIN155169) detektiert spezifisch Podoplanin in WB, IP, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für Podoplanin Antikörper (ABIN155169)

Target: Podoplanin (PDPN)

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RTD4E10

Produktdetails anti-Podoplanin Antikörper

Spezifität: The monoclonal antibody will detect Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. The antibody does not cross-react with Human Podoplanin.

Aufreinigung: Protein G Chromatography

Immunogen: Murine thymic stromal cell line

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (use diluted 1-10 μg/mL IgG). Works well with paraffin sections. Western blot (use 1-10 μg/mL IgG, will detect a 36-40 kDa band). Immunoprecipitation (use 1-5 μg IgG per 1 mLlysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4, without preservatives or stabilizers

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. After reconstitution store the antibody (in aliquots) at -20 °C.

Referenzen für anti-Podoplanin Antikörper (ABIN155169)

Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." in: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).

DAmico, Jones, Nye, Sapienza, Ramjuan, Reynolds, Robinson, Kostourou, Martinez, Aubyn, Grose, Thomas, Spencer-Dene, Zicha, Davies, Tybulewicz, Hodivala-Dilke: "Regulation of lymphatic-blood vessel separation by endothelial Rac1." in: Development (Cambridge, England), Vol. 136, Issue 23, pp. 4043-53, (2009) (PubMed).

NOYES, DOMM, WILLSLC: "Regulation of erythropoiesis. I. Erythropoietin assay as a clinical tool." in: Blood, Vol. 20, pp. 9-18 (PubMed).

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: Podoplanin

Hintergrund: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gen-ID: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport