FLT1 Antikörper (Extracellular Domain) (Biotin)

Produktnummer ABIN155162

Der Maus-Monoklonal Anti-FLT1-Antikörper wurde für WB, IP, IF und EIA validiert. Er ist geeignet zum Nachweis von FLT1 in Proben aus Human und Maus. }

Kurzübersicht für FLT1 Antikörper (Extracellular Domain) (Biotin) (ABIN155162)

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FLT1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Klon: FLTEWF

Produktdetails anti-FLT1 Antikörper (Biotin)

Bindungsspezifität: Extracellular Domain

Spezifität: The unconjugated monoclonal antibody will recognize recombinant and naturally occurring form of VEGFR-1/Flt-1.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse.

Aufreinigung: Protein G Chromatography

Immunogen: Recombinant Human soluble extracellular Flt-1 protein (D5)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (undiluted 1.5 - 8 μg/mL). Western blotting (1 - 10 μg/mL). Immunofluorescence. Immunoprecipitation (1 - 5 μg/mL lysate or reaction volume).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, 0.02 % Sodium Azide, 50X BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FLT1

Target: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Andere Bezeichnung: VEGFR-1 / Flt-1

Hintergrund: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor

Gen-ID: 2321

NCBI Accession: NP_001153392

UniProt: P17948

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals