Prostate Specific Antigen Antikörper

Produktnummer ABIN1543584

Dieses Anti-Prostate Specific Antigen-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Prostate Specific Antigen in ELISA, WB, FACS und DB. Geeignet für Human. Unabhängig validiert für die Verwendung in Flow Cytometry.

Kurzübersicht für Prostate Specific Antigen Antikörper (ABIN1543584)

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Klon: A5D5-1

Produktdetails anti-Prostate Specific Antigen Antikörper

Aufreinigung: Protein A affinity column

Immunogen: Native PSA Antigen

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Other Applications have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (FACS)

Validierung #029617 (Flow Cytometry)

by: Flow Cytometry & Cell Separation Facility, Purdue University

No.: #029617

Datum: 22.02.2014

Chargennummer: FAPS-A5D5-0003

Validierte Anwendung: Flow Cytometry

Positivkontrolle: LNCaP cells

Negativkontrolle: - DU145 cells - Jurkat cells

Bewertung: Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.

Validierungsbilder

Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).

Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).

Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).

Protokoll

Primärantikörper:

• Antibody: Prostate Specific Antigen (PSA)
• Catalog number: ABIN1543584
• Lot number: FAPS-A5D5-0003

Sekundärantikörper:

• Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647

Full Protocol:

• Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
• Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
• An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
• Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
• Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
• 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
• Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
• Cells were washed twice with PBS/BSA/Tween 20
• Cells were resuspended in 0.3 mL PBS/BSA
• Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Anmerkungen: None

Handhabung

Format: Liquid

Konzentration: 1.8 mg/mL

Buffer: PBS, pH 7.4, 0.09% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: PSA

Pathways: Komplementsystem