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Prostate Specific Antigen Antikörper

Dieses Anti-Prostate Specific Antigen-Antikörper ist ein Mouse Monoclonal-Antikörper zur Detektion von Prostate Specific Antigen in ELISA, WB, FACS, DB. Geeignet für Human. Unabhängig validiert für die Verwendung in Flow Cytometry.
Produktnummer ABIN1543584

Kurzübersicht für Prostate Specific Antigen Antikörper (ABIN1543584)

Target

Alle Prostate Specific Antigen (PSA) Antikörper anzeigen
Prostate Specific Antigen (PSA)

Reaktivität

  • 175
  • 13
  • 6
  • 4
  • 1
  • 1
  • 1
Human

Wirt

  • 91
  • 83
  • 4
  • 3
  • 2
Maus

Klonalität

  • 108
  • 76
Monoklonal

Konjugat

  • 129
  • 11
  • 10
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation

  • 82
  • 68
  • 61
  • 33
  • 25
  • 24
  • 24
  • 23
  • 12
  • 12
  • 10
  • 7
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB)

Klon

A5D5-1
  • Aufreinigung

    Protein A affinity column

    Immunogen

    Native PSA Antigen

    Isotyp

    IgG1
  • Kommentare

    Other Applications have not been tested.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #029617 (Flow Cytometry)
    'Independent Validation' Siegel
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029617
    Datum
    22.02.2014
    Antigen
    Chargennummer
    FAPS-A5D5-0003
    Validierte Anwendung
    Flow Cytometry
    Positivkontrolle
    LNCaP cells
    Negativkontrolle
    - DU145 cells - Jurkat cells
    Bewertung
    Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: Prostate Specific Antigen (PSA)
    • Catalog number: ABIN1543584
    • Lot number: FAPS-A5D5-0003
    Sekundärantikörper
    • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
    Full Protocol
    • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
    • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
    • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 0.3 mL PBS/BSA
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Anmerkungen
    None
  • Format

    Liquid

    Konzentration

    1.8 mg/mL

    Buffer

    PBS, pH 7.4, 0.09% sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C
  • Target

    Prostate Specific Antigen (PSA)

    Andere Bezeichnung

    PSA

    Pathways

    Komplementsystem
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