Prostate Specific Antigen Antikörper
Kurzübersicht für Prostate Specific Antigen Antikörper (ABIN1543584)
Target
Alle Prostate Specific Antigen (PSA) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Aufreinigung
- Protein A affinity column
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Immunogen
- Native PSA Antigen
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Isotyp
- IgG1
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Kommentare
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Other Applications have not been tested.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- by
- Flow Cytometry & Cell Separation Facility, Purdue University
- No.
- #029617
- Datum
- 22.02.2014
- Antigen
- Chargennummer
- FAPS-A5D5-0003
- Validierte Anwendung
- Flow Cytometry
- Positivkontrolle
- LNCaP cells
- Negativkontrolle
- - DU145 cells - Jurkat cells
- Bewertung
- Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
- Primärantikörper
- Antibody: Prostate Specific Antigen (PSA)
- Catalog number: ABIN1543584
- Lot number: FAPS-A5D5-0003
- Sekundärantikörper
- Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
- Full Protocol
- Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
- Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
- An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
- Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
- Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
- Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
- 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
- Cells were washed twice with PBS/BSA/Tween 20
- Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
- Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
- Cells were washed twice with PBS/BSA/Tween 20
- Cells were resuspended in 0.3 mL PBS/BSA
- Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
- Anmerkungen
- None
Validierung #029617 (Flow Cytometry)ValidierungsbilderProtokoll -
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Format
- Liquid
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Konzentration
- 1.8 mg/mL
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Buffer
- PBS, pH 7.4, 0.09% sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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- Prostate Specific Antigen (PSA)
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Andere Bezeichnung
- PSA
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Pathways
- Komplementsystem
Target
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