APAF1 Antikörper (AA 289-338)

Produktnummer ABIN1533212

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch APAF1 in WB, IHC, ELISA und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für APAF1 Antikörper (AA 289-338) (ABIN1533212)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APAF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-APAF1 Antikörper

Bindungsspezifität: AA 289-338

Verwendungszweck: Apaf-1-ALT Antibody

Spezifität: Apaf-1-ALT Polyclonal Antibody detects endogenous levels of Apaf-1-ALT protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human APAF-1-ALT. AA range:289-338

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Hintergrund:

Synonyms: APAF1-interacting protein, APIP, APIP2, CGI-29 protein, MMRP19, dJ179L10.2, likely ortholog of mouse monocyte macrophage 19

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Gene Name: APAF1

Protein Name: Apoptotic protease-activating factor 1

Molekulargewicht: 40 kDa

Gen-ID: 317

OMIM: 602233

UniProt: O14727-6

Pathways: p53 Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Tube Formation, Positive Regulation of Endopeptidase Activity