MDM4-binding Protein Antikörper (pSer367)

Produktnummer ABIN1532152

Der Kaninchen Polyklonal anti-MDM4-binding Protein Antikörper wird verwendet zum Nachweis von MDM4-binding Protein in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für MDM4-binding Protein Antikörper (pSer367) (ABIN1532152)

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDM4-binding Protein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MDM4-binding Protein Antikörper

Bindungsspezifität: AA 336-385, pSer367

Verwendungszweck: MDMX (phospho Ser367) Antibody

Spezifität: Phospho-MDMX (S367) Polyclonal Antibody detects endogenous levels of MDMX protein only when phosphorylated at S367.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MDM4 around the phosphorylation site of Ser367. AA range:336-385

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Andere Bezeichnung: MDM4

Hintergrund:

Synonyms: MDM4; MDMX; Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4

Background: This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011],

Gene Name: MDM4

Protein Name: Protein Mdm4

Molekulargewicht: 54 kDa

Gen-ID: 4194

OMIM: 602704

UniProt: O15151

Pathways: Zellzyklus