Vinculin Antikörper (pTyr821)

Produktnummer ABIN1531640

Der Kaninchen Polyklonal anti-Vinculin Antikörper wird verwendet zum Nachweis von Vinculin in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA und IF.

Kurzübersicht für Vinculin Antikörper (pTyr821) (ABIN1531640)

Target: Vinculin (VCL)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Vinculin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-Vinculin Antikörper

Bindungsspezifität: AA 786-835, pTyr821

Verwendungszweck: Vinculin (phospho Tyr821) Antibody

Spezifität: Phospho-Vinculin (Y821) Polyclonal Antibody detects endogenous levels of Vinculin protein only when phosphorylated at Y821.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: Vinculin

Hintergrund:

Synonyms: VCL, Vinculin, Metavinculin

Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Gene Name: VCL

Protein Name: Vinculin

Molekulargewicht: 123 kDa

Gen-ID: 7414

OMIM: 193065

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2