KSR1 Antikörper (pSer392)

Produktnummer ABIN1531231

Der Kaninchen Polyklonal anti-KSR1 Antikörper wird verwendet zum Nachweis von KSR1 in Proben von Human und Maus. Er wurde validiert für WB, ELISA, IHC und IF.

Kurzübersicht für KSR1 Antikörper (pSer392) (ABIN1531231)

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KSR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-KSR1 Antikörper

Bindungsspezifität: AA 358-407, pSer392

Verwendungszweck: Ksr-1 (phospho Ser392) Antibody

Spezifität: Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human KSR around the phosphorylation site of Ser392. AA range:358-407

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Andere Bezeichnung: KSR

Hintergrund:

Synonyms: KSR1, KSR, Kinase suppressor of Ras 1

Background: caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.,PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.,subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.,

Gene Name: KSR1

Protein Name: Kinase suppressor of Ras 1

Molekulargewicht: 102 kDa

Gen-ID: 8844

OMIM: 601132

UniProt: Q8IVT5