AMPK alpha Antikörper (pThr172)

Produktnummer ABIN1531172

Der Kaninchen Polyklonal anti-AMPK alpha Antikörper (ABIN1531172) detektiert spezifisch AMPK alpha in WB, IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Affe, Schwein und Shrimp.

Kurzübersicht für AMPK alpha Antikörper (pThr172) (ABIN1531172)

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Reaktivität: Human, Maus, Ratte, Affe, Schwein, Shrimp

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AMPK alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-AMPK alpha Antikörper

Bindungsspezifität: AA 140-189, pThr172

Verwendungszweck: AMPKα1/2 (phospho Thr183/172) Antibody

Spezifität: Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu AMPK alpha

Target: AMPK alpha (SNF1A) (SNF1A/AMP-Activated Protein Kinase (SNF1A))

Andere Bezeichnung: AMPK alpha

Hintergrund:

Synonyms: PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

Gene Name: AAPK1/AAPK2

Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1/2

Molekulargewicht: 62 kDa

Gen-ID: 5562, 5563

OMIM: 602739

UniProt: Q13131

Pathways: Warburg Effekt