SPARC Antikörper

Produktnummer ABIN1450173

Der Maus Monoklonal anti-SPARC Antikörper (Klon N50) (ABIN1450173) detektiert spezifisch SPARC in WB, IHC (p), IF, FACS, IHC (fro), EIA und RIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Rind (Kuh).

Kurzübersicht für SPARC Antikörper (ABIN1450173)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reaktivität: Human, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA)

Klon: N50

Produktdetails anti-SPARC Antikörper

Spezifität: Recognizes Human and Bovine bone Osteonectin, Human platelet Osteonectin. SPARC produced by various cells grown in culture. Cross reacts with Rat Osteonectin.Aff. Const.:5 x 10e-9 M

Aufreinigung: Ammonium Sulfate Precipitation, Ion Exchange Chromatography and Gel Filtration.

Immunogen: Crude bone extract containing native Osteonectin.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 10.8 mg/mL (OD280 nm, E1% = 14)

Buffer: 50 % Glycerol/H2O (vol/vol), None

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC / Osteonectin

Hintergrund: SPARC (secreted protein acidic and rich in cysteine)/Osteonectin is a matricellular glycoprotein that modulates cellular interactions with the ECM and is expressed in tissues undergoing remodeling. It functions as a de-adhesive protein, as a modulator of growth factor activity, and as a cell-cycle inhibitor. It induces changes in endothelial cell shape via F-actin, coincident with the appearance of intercellular gaps, that provide a paracellular pathway for extravasation of macromolecules. Tumor growth is enhanced in mice lacking SPARC due to changes in the ECM that create a more permissive environment for tumor progression.Synonyms: BM-40, Basement-membrane protein 40, ON, Secreted protein acidic and rich in cysteine

Gen-ID: 6678

NCBI Accession: NP_003109

UniProt: P09486

Pathways: Autophagie