ATG4D Antikörper (AA 227-257)

Produktnummer ABIN1449634

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATG4D in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für ATG4D Antikörper (AA 227-257) (ABIN1449634)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG4D Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ATG4D Antikörper

Bindungsspezifität: AA 227-257

Aufreinigung: Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 227~257 amino acids from the Center of Human APG4D Genename: ATG4D

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Andere Bezeichnung: APG4D / ATG4D

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.Synonyms: AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D

Molekulargewicht: 52922 Da

Gen-ID: 84971

NCBI Accession: NP_116274

Pathways: Autophagie