MED7 Antikörper (AA 101-200) (Cy3)

Produktnummer ABIN1409103

Dieses Anti-MED7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MED7 in WB, IF (cc) und IF (p). Geeignet für Human.

Kurzübersicht für MED7 Antikörper (AA 101-200) (Cy3) (ABIN1409103)

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED7 Antikörper ist konjugiert mit Cy3

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-MED7 Antikörper (Cy3)

Bindungsspezifität: AA 101-200

Homologie: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Zebrafish

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CRSP9

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MED7

Target: MED7 (Mediator Complex Subunit 7 (MED7))

Andere Bezeichnung: CRSP9

Hintergrund:

Synonyms: Cofactor required for Sp1 transcriptional activation subunit 9, CRSP complex subunit 9, CRSP33, hMED7, MED7, MED7_HUMAN, Mediator complex subunit 7, Mediator of RNA polymerase II transcription subunit 7, MGC12284, RNA polymerase transcriptional regulation mediator subunit 7 homolog, Transcriptional coactivator CRSP33, Activator recruited cofactor 34 kDa component, Activator-recruited cofactor 34 kDa component, ARC34.

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase (1). These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes, and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34) (2,3). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors (4). Med6 and Med7, as well as other components of SMCC, associate with coactivator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation (4,5). Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity (6).

Gen-ID: 9443

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha