RUNX1 Antikörper (pSer249) (FITC)

Produktnummer ABIN1396162

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RUNX1 in WB, IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für RUNX1 Antikörper (pSer249) (FITC) (ABIN1396162)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RUNX1 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-RUNX1 Antikörper (FITC)

Bindungsspezifität: pSer249

Kreuzreaktivität: Maus

Homologie: Human,Rat,Dog,Cow,Pig,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RUNX1 around the phosphorylation site of Ser249 [QP(p-S)PP]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Andere Bezeichnung: RUNX1

Hintergrund:

Synonyms: RUNX1 Ser249, p-RUNX1 Ser249, RUNX1 phospho S249, RUNX1 phospho Ser249, Acute myeloid leukemia 1, Acute myeloid leukemia 1 protein, alpha subunit core binding factor, AML 1, AML1 EVI 1, AML1, Aml1 oncogene, AMLCR 1, AMLCR1, CBFA 2, CBFA2, Core binding factor alpha 2 subunit, Core binding factor runt domain alpha subunit 2, EVI 1, EVI1, HGNC, Oncogene AML 1, PEA2 alpha, PEBP2 alpha B, PEBP2A2, PEBP2aB, Polyomavirus enhancer binding protein 2 alpha B subunit, Run1, Runt related transcription factor 1, RUNX 1, SL3 3 enhancer factor 1 alpha B subunit, SL3/AKV core binding factor alpha B subunit, RUNX1_HUMAN.

Background: AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in a wide variety of tissues and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.