LEF1 Antikörper (Lymphoid Enhancer-Binding Factor 1) (pSer42)

Details for Product anti-LEF1 Antibody No. ABIN1387756, Anbieter: Anmelden zum Anzeigen
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer-binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • lef1
  • Lef1
Epitop
pSer42
14
14
13
9
8
6
6
5
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
130
41
29
11
7
7
7
5
5
4
4
4
3
2
1
1
Wirt
Kaninchen
56
54
16
5
Klonalität
Polyklonal
Konjugat
Dieser LEF1 Antikörper ist unkonjugiert
3
3
3
3
3
3
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
67
23
20
9
8
4
4
2
2
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Isotyp IgG
Spezifität This antibody will recognize LEF1(Ser40) in mouse and rat
Reinigung Purified by Protein A.
Andere Bezeichnung LEF1 (LEF1 Antibody Abstract)
Hintergrund

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Subcellular location: Nucleus

Synonyms: LEF-1, TCF10, TCF7L3, TCF1ALPHA, Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, TCF1-alpha, LEF1

Gen-ID 51176
UniProt Q9UJU2
Pathways WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (pSer42) antibody (ABIN1387756) Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-...
Produkt verwendet in: Fu, Huang, Wang, Chen, Huang, Lu, Liang, Zhang: "Proteome Profile and Quantitative Proteomic Analysis of Buffalo (Bubalusbubalis) Follicular Fluid during Follicle Development." in: International journal of molecular sciences, Vol. 17, Issue 5, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Others). Weitere Details: Sample: Ovaries

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