MHC Class II Antikörper (PE)

Produktnummer ABIN135569

Der Maus Monoklonal Anti-MHC Class II-Antikörper wurde für FACS validiert. Er ist geeignet, MHC Class II in Proben von Huhn zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für MHC Class II Antikörper (PE) (ABIN135569)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reaktivität: Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: 2G11

Produktdetails anti-MHC Class II Antikörper (PE)

Spezifität: Chicken/Pigeon/Caiman MHC Class II β-chain, Mr 30-42 kDa

Produktmerkmale: Mouse Anti-Chicken MHC Class II-PE

Aufreinigung: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Unknown

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen für anti-MHC Class II Antikörper (PE) (ABIN135569)

Weiss, Christensen, Zeuthen, Vogensen, Jakobsen, Frøkiær: "Lactobacilli and bifidobacteria induce differential interferon-β profiles in dendritic cells." in: Cytokine, Vol. 56, Issue 2, pp. 520-30, (2011) (PubMed).

Details zu MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Andere Bezeichnung: MHC Class II

Hintergrund: The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).