CD8B Antikörper (FITC)

Produktnummer ABIN135542

Der Maus Monoklonal Anti-CD8B-Antikörper wurde für FACS validiert. Er ist geeignet, CD8B in Proben von Huhn zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für CD8B Antikörper (FITC) (ABIN135542)

Target: CD8B (CD8b Molecule (CD8B))

Reaktivität: Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8B Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: EP42

Produktdetails anti-CD8B Antikörper (FITC)

Spezifität: Chicken CD8β, Mr 34 kDa

Produktmerkmale: Mouse Anti-Chicken CD8b-FITC

Immunogen: Chicken splenocytes

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen für anti-CD8B Antikörper (FITC) (ABIN135542)

Lacharme-Lora, Chaloner, Gilroy, Humphrey, Gibbs, Jopson, Wright, Reid, Ketley, Humphrey, Williams, Rushton, Wigley: "B lymphocytes play a limited role in clearance of Campylobacter jejuni from the chicken intestinal tract." in: Scientific reports, Vol. 7, pp. 45090, (2018) (PubMed).

Dalgaard, Norup, Pedersen, Handberg, Juorgensen, Juul-Madsen: "Flow cytometric assessment of chicken T cell-mediated immune responses after Newcastle disease virus vaccination and challenge." in: Vaccine, (2010) (PubMed).

Details zu CD8B

Target: CD8B (CD8b Molecule (CD8B))

Andere Bezeichnung: CD8b

Hintergrund: In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two chains, and (ii) a heterodimer of an chain and a chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8- and CD8-chains, a relatively large proportion of the CD8+ TCR- cells in the spleens of embryos and young chicks express only the -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- CD8 cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80 % of thymocytes, 45 % of blood mononuclear cells and 50 % of spleen cells, but <1 % of cells in the bursa and bone marrow.

Pathways: T-Zell Rezeptor Signalweg