CD86 Antikörper

Produktnummer ABIN135273

Dieses Anti-CD86-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD86 in FACS. Geeignet für Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für CD86 Antikörper (ABIN135273)

Target: CD86

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD86 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: GL1

Produktdetails anti-CD86 Antikörper

Spezifität: Mouse CD86, Mr 80 kDa

Produktmerkmale: Rat Anti-Mouse CD86-UNLB

Aufreinigung: Purified

Immunogen: LPS-activated CBA/Ca mouse spleen B cells

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY7, AF647, and AF700 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Kommentare:

In vivo and in vitro blocking assays

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Konservierungsmittel: Without preservative

Handhabung: Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C

Referenzen für anti-CD86 Antikörper (ABIN135273)

Ma, Martínez-Ramírez, Borders, Gao, Sosa-Pineda: "Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts." in: eLife, Vol. 9, (2020) (PubMed).

Details zu CD86

Target: CD86

Andere Bezeichnung: CD86

Hintergrund: CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation. CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation