Phosphotyrosine Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN135059

Der Maus Monoklonal Anti-Phosphotyrosine-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, Phosphotyrosine in Proben von zu detektieren.

Kurzübersicht für Phosphotyrosine Antikörper (Alkaline Phosphatase (AP)) (ABIN135059)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist konjugiert mit Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: PY20

Produktdetails anti-Phosphotyrosine Antikörper (Alkaline Phosphatase (AP))

Produktmerkmale: The antibody detects: Phosphorylated tyrosine and phenylphosphate

Aufreinigung: Purified

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Working Dilution:
ELISA: 1:500-1:1,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Probenmenge: 1 mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Product is supplied as 1.0 mL of stock solution in 50 mM Tris / 0 mM MgCl2 / 50 % Glycerol, pH 8.0, containing 0.1 % NaN3 as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.