Notch1 Antikörper (Extracellular, N-Term)

Produktnummer ABIN1304873

Der Maus Monoklonal Anti-Notch1 Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, Notch1 in Proben von Maus zu detektieren.

Kurzübersicht für Notch1 Antikörper (Extracellular, N-Term) (ABIN1304873)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Klon: N253-32

Produktdetails anti-Notch1 Antikörper

Bindungsspezifität: AA 20-216, Extracellular, N-Term

Verwendungszweck: Anti-Notch1 Antibody

Spezifität: No off-targets reported for Notch2 or Notch3

Kreuzreaktivität: Ratte

Produktmerkmale: Our Anti-Notch1 mouse monoclonal primary antibody is produced in-house from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Aufreinigung: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Reinheit: Purified mAbs are >90 % specific antibody.

Immunogen: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 (accession number Q01705) produced recombinantly in E. Coli

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 10 mM Tris, 50 mM Sodium Chloride, 0.065 % Sodium Azide 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Haltbarkeit: 24 months

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch1

Hintergrund:

Synonyms: Neurogenic locus notch homolog protein 1 (Notch 1) (Motch A) (mT14) (p300) [Cleaved into: Notch 1 extracellular truncation (NEXT), Notch 1 intracellular domain (NICD)]

Description Logic: Our Anti-Notch1 mouse monoclonal primary antibody is produced from hybridoma clone N253/32. It detects mouse and rat Notch1, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Gene Name Alternatives: Notch1 Motch

Molekulargewicht: >270 kDa, ~120 kDa and smaller fragments (due to proteolysis)

UniProt: Q01705

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development