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KCNQ4 Antikörper (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4) Primary Antibody

KCNQ4 Reaktivität: Säugetier ISt, IHC, WB Wirt: Maus Monoclonal N43-6 unconjugated
Pubmed (1 reference) Independent Validation (1 Validierung)
Produktnummer ABIN1304779
$460.17
Zzgl. Versandkosten $45.00
100 μL
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  • Target Alle KCNQ4 Antikörper anzeigen
    KCNQ4
    Reaktivität
    • 52
    • 25
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Säugetier
    Wirt
    • 28
    • 18
    • 7
    Maus
    Klonalität
    • 36
    • 17
    Monoklonal
    Konjugat
    • 26
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser KCNQ4 Antikörper ist unkonjugiert
    Applikation
    • 42
    • 24
    • 21
    • 17
    • 14
    • 13
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunostaining (ISt), Immunohistochemistry (IHC), Western Blotting (WB)
    Produktmerkmale
    K+ channels
    Aufreinigung
    Purified: Hybridoma in vivo ascites fluid purified by SAS (Saturated Ammonium Sulfate) precipitation followed by anion exchange chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.
    Klon
    N43-6
    Isotyp
    IgG1
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102037 (Western Blotting)
    'Independent Validation' Siegel
    by
    Dipartimento di Medicina e Scienze della Salute, Università degli Studi del Molise, Campobasso
    No.
    #102037
    Datum
    31.10.2017
    Antigen
    Kv7.4/KCNQ4
    Chargennummer
    440-2HK-65b
    Validierte Anwendung
    Western Blotting
    Positivkontrolle

    CHO cells transfected with a plasmid containing the cDNA for the longest isoform of the human Kv7.4/KCNQ4 channel

    Negativkontrolle

    Non-transfected CHO cells (no Kv7 channel expression)

    Bewertung

    Passed. ABIN1304779 specifically detects KCNQ4/Kv7.4 in lysates from CHO cells expressing human KCNQ4/Kv7.4.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN654696
    Sekundärantikörper
    goat anti-mouse IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013)
    Full Protocol
    • Grow CHO cells (Chinese Hamster Ovary cells) in DMEM medium (Microgem, L0106-500) supplemented with 10% fetal bovine serum (Microgem, S1810-500) and antibiotics (1X Penicillin-Streptomycin mix, Microgem, L0022-100), at 37°C and 5% CO2 in 60mm plates.
    • Transfect CHO cells with 5µg plasmid expressing either Kv7.1, Kv7.2, Kv7.3, Kv7.4, or Kv7.5 and 1µg of plasmid expressing EGFP as a transfection marker using Lipofectamine 2000 (Invitrogen, 11668019) following the manufacturer´s instructions.
    • Lyse cells in an appropriate volume of B-buffer, containing: 150mM NaCl, 10mM Tris-HCl pH7.4, 1mM EDTA, 1% SDS and 1x protease inhibitors (Roche, 11836145001, lot 11231400).
    • Determine total protein content of the lysates using a Bradford protein assay (AppliChem, A6932, lot 6J015116).
    • Denature 20µg of total protein for 10min at 100°C in 1/3 volume of 4x Laemmli SDS sample buffer and subsequently separate them on a denaturing 8% SDS-PAGE gel in Mini-PROTEAN Electrophoresis Cells (Bio-Rad) for 2-3h at 100 V.
    • Transfer proteins onto PVDF filter (Bio-Rad, 1620177, lot 28369A01) with transfer buffer (25mM Tris, 192mM glycine, 20% methanol) in a western blotting system at 4°C at 40mA ON.
    • Block the membrane with blocking buffer (5% milk dissolved in PBS + 1% Tween20, PBS-T) for 1h at RT.
    • Incubation with primary
      • monoclonal mouse anti-KCNQ4/Kv7.4 antibody (antibodies-online, ABIN1304779, lot 440-2HK-65b) diluted 1:1000 in blocking buffer ON at 4°C or
      • mouse anti-alpha-tubulin (Sigma, T8203, lot 024M4827V) diluted 1:5000 in blocking buffer ON at 4°C.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubation with secondary goat anti-mouse IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013) diluted 1:5000 in blocking buffer for 1h at RT.
    • Wash membrane 3x for 10min with PBS-T.
    • Treat PVDF filters with chemiluminescence solutions (ECL, Promega) for 5min at RT.
    • Images were captured, stored, and analyzed with the ImageLab, version 4.1 analysis software (Bio-Rad).
    Anmerkungen
  • Format
    Liquid
  • Testai, Barrese, Soldovieri, Ambrosino, Martelli, Vinciguerra, Miceli, Greenwood, Curtis, Breschi, Sisalli, Scorziello, Canduela, Grandes, Calderone, Taglialatela: "Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection." in: Cardiovascular research, Vol. 110, Issue 1, pp. 40-50, (2016) (PubMed).

  • Target
    KCNQ4
    Andere Bezeichnung
    Kv7.4/KCNQ4 (KCNQ4 Produkte)
    Synonyme
    KCNQ4, k(v)7.4, kv7.4, DFNA2, DFNA2A, KV7.4, potassium voltage-gated channel subfamily Q member 4, potassium channel, voltage gated KQT-like subfamily Q, member 4, potassium voltage-gated channel, subfamily Q, member 4, KCNQ4, kcnq4, Kcnq4
    UniProt
    P56696
    Pathways
    Sensory Perception of Sound
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