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GAPDHS Antikörper (FITC)

Der Maus Monoklonal Anti-GAPDHS-Antikörper wurde für FACS validiert. Er ist geeignet, GAPDHS in Proben von Human und Schwein zu detektieren.
Produktnummer ABIN1302845

Kurzübersicht für GAPDHS Antikörper (FITC) (ABIN1302845)

Target

Alle GAPDHS Antikörper anzeigen
GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reaktivität

  • 23
  • 18
  • 17
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Human, Schwein

Wirt

  • 31
  • 7
  • 1
Maus

Klonalität

  • 33
  • 6
Monoklonal

Konjugat

  • 18
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GAPDHS Antikörper ist konjugiert mit FITC

Applikation

  • 32
  • 13
  • 13
  • 7
  • 6
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Flow Cytometry (FACS)

Klon

Hs-8
  • Verwendungszweck

    Anti-GAPDHS FITC

    Spezifität

    The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

    Kreuzreaktivität (Details)

    Human, Porcine

    Aufreinigung

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    Immunogen

    Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

    Isotyp

    IgM
  • Applikationshinweise

    Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid prolonged exposure to light.
    Do not use after expiration date stamped on vial label.
    Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target

    GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

    Andere Bezeichnung

    GAPDHS

    Hintergrund

    Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35

    Gen-ID

    26330

    UniProt

    O14556

    Pathways

    Regulation of Carbohydrate Metabolic Process
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