VCP Antikörper (Valosin Containing Protein) (FITC)

Produktnummer ABIN1302844

Produktdetails anti-VCP Antikörper

Target: VCP

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VCP Antikörper ist konjugiert mit FITC

Applikation: Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)

Spezifität: The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".

Kreuzreaktivität (Details): Mouse, Human

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Klon: Hs-14

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of sperm cells using 20 μ,l reagent / 100 μ,l cell suspension.The content of a vial (1 ml) is sufficient for 50 tests.

Kommentare:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Referenzen für anti-VCP Antikörper (ABIN1302844)

Peknicova, Chladek, Hozak: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).

Chládek, P?knicová, Capková, Geussová, Teplá, Madar: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).

Details zu VCP

Target: VCP

Andere Bezeichnung: VCP (VCP Produkte)

Synonyme: ALS14, IBMPFD, TERA, p97, CDC48, wu:fd16d05, wu:fj63d11, p93, 3110001E05, p97/VCP, 22.26, 7.12, Apple, BcDNA.GM02885, BcDNA:GM02885, CG2331, DmTER94, Dmel\CG2331, I, Ter94, Tera, VCP, VCP ATPase, VCP/p97, Vcp, anon-WO2004063362.65, anon-WO2004063362.67, cdc48, dTERA, dVCP, dmTERA, dvcp, l(2)03775, l(2)46CFf, l(2)46CFs, l(2)46Ch, pTER94, ter94, vcp, FANCG, valosin containing protein, valosin containing protein S homeolog, valosin-containing protein, CG2331 gene product from transcript CG2331-RA, transitional endoplasmic reticulum ATPase, VCP, vcp.S, vcp, Vcp, Bm1_56585, LOC100136246, TER94, LOC101109680

Hintergrund: VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway