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VCP Antikörper (Valosin Containing Protein) (FITC) Primary Antibody

VCP Reaktivität: Human, Maus ICC, FACS, WB Wirt: Maus Monoclonal Hs-14 FITC
Pubmed (2 references)
Produktnummer ABIN1302844
$567.73
Zzgl. Versandkosten $45.00
50 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 5 bis 7 Werktagen
  • Target Alle VCP Antikörper anzeigen
    VCP
    Reaktivität
    • 99
    • 46
    • 42
    • 16
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 81
    • 21
    • 2
    Maus
    Klonalität
    • 74
    • 30
    Monoklonal
    Konjugat
    • 63
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Dieser VCP Antikörper ist konjugiert mit FITC
    Applikation
    • 80
    • 50
    • 28
    • 20
    • 19
    • 14
    • 11
    • 10
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
    Spezifität
    The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein".
    Kreuzreaktivität (Details)
    Mouse, Human
    Immunogen
    Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
    Klon
    Hs-14
    Isotyp
    IgM
  • Applikationshinweise
    The reagent is designed for Flow Cytometry analysis of sperm cells using 20 μ,l reagent / 100 μ,l cell suspension.The content of a vial (1 ml) is sufficient for 50 tests.
    Kommentare

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    No reconstitution is necessary.
    Buffer
    The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Avoid prolonged exposure to light.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
  • Peknicova, Chladek, Hozak: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).

    Chládek, P?knicová, Capková, Geussová, Teplá, Madar: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).

  • Target
    VCP
    Andere Bezeichnung
    VCP (VCP Produkte)
    Synonyme
    ALS14, IBMPFD, TERA, p97, CDC48, wu:fd16d05, wu:fj63d11, p93, 3110001E05, p97/VCP, 22.26, 7.12, Apple, BcDNA.GM02885, BcDNA:GM02885, CG2331, DmTER94, Dmel\CG2331, I, Ter94, Tera, VCP, VCP ATPase, VCP/p97, Vcp, anon-WO2004063362.65, anon-WO2004063362.67, cdc48, dTERA, dVCP, dmTERA, dvcp, l(2)03775, l(2)46CFf, l(2)46CFs, l(2)46Ch, pTER94, ter94, vcp, FANCG, valosin containing protein, valosin containing protein S homeolog, valosin-containing protein, CG2331 gene product from transcript CG2331-RA, transitional endoplasmic reticulum ATPase, VCP, vcp.S, vcp, Vcp, Bm1_56585, LOC100136246, TER94, LOC101109680
    Hintergrund
    VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
    Pathways
    ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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