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LIME Antikörper (AA 86-202)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch LIME in WB und IP. Er zeigt eine Reaktivität gegenüber Maus.
Produktnummer ABIN1302445

Kurzübersicht für LIME Antikörper (AA 86-202) (ABIN1302445)

Target

Alle LIME (LIME1) Antikörper anzeigen
LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reaktivität

  • 25
  • 2
Maus

Wirt

  • 18
  • 8
  • 1
Maus

Klonalität

  • 20
  • 7
Monoklonal

Konjugat

  • 17
  • 3
  • 2
  • 2
  • 2
  • 1
Dieser LIME Antikörper ist unkonjugiert

Applikation

  • 19
  • 11
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP)

Klon

MLIME-05
  • Bindungsspezifität

    • 9
    • 6
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 86-202

    Verwendungszweck

    Anti-Ms Lime1 Purified

    Spezifität

    Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

    Kreuzreaktivität (Details)

    Mouse, Other not tested

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Recombinant protein corresponding to amino acids 86-202 of mouse LIME

    Isotyp

    IgG2a
  • Applikationshinweise

    Immunoprecipitation: Positive control: murine splenic T cells.
    Western blotting: Reducing conditions, recommended dilution: 1-5 μg/mL.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Target

    LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

    Andere Bezeichnung

    Lime1

    Hintergrund

    Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,Lita1

    Gen-ID

    72699

    UniProt

    Q9EQR5
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