LIME Antikörper (AA 86-202)

Produktnummer ABIN1302445

Produktdetails anti-LIME Antikörper

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Bindungsspezifität: AA 86-202

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIME Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Verwendungszweck: Anti-Ms Lime1 Purified

Spezifität: Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Kreuzreaktivität (Details): Mouse, Other not tested

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Klon: MLIME-05

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Andere Bezeichnung: Lime1 (LIME1 Produkte)

Hintergrund: Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,Lita1

Gen-ID: 72699

UniProt: Q9EQR5