ABCB1 Antikörper (AA 255-280)

Produktnummer ABIN129711

Hersteller Produkt- Nr.: 600-401-967

Produktdetails anti-ABCB1 Antikörper

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Bindungsspezifität: AA 255-280

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ABCB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Hersteller Produkt- Nr.: 600-401-967

Hersteller: Rockland

Verwendungszweck: ABCB1 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against human ABCB1 protein.

Produktmerkmale: Synonyms: rabbit anti-ABCB1 Antibody, ABCB 1, ABCB-1, ATP binding cassette sub family B MDR/TAP member 1 antibody, CD243 antibody, CD243 antigen antibody, CLCS antibody, GP170 antibody, MDR 1 antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 255-280 of human ABCB1 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-ABCB1 Antikörper (ABIN129711)

Al-Shawi, Omote: "The remarkable transport mechanism of P-glycoprotein: a multidrug transporter." in: Journal of bioenergetics and biomembranes, Vol. 37, Issue 6, pp. 489-96, (2006) (PubMed).

Details zu ABCB1

Target: ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))

Andere Bezeichnung: ABCB1 (ABCB1 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.

Gen-ID: 5243, 42741659

UniProt: P08183