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SPRY4 Antikörper (AA 275-299)

SPRY4 Reaktivität: Human, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN129626
Hersteller Produkt- Nr.: 600-401-697
  • Target Alle SPRY4 Antikörper anzeigen
    SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
    Bindungsspezifität
    • 8
    • 7
    • 7
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 275-299
    Reaktivität
    • 49
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 49
    Kaninchen
    Klonalität
    • 47
    • 2
    Polyklonal
    Konjugat
    • 24
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SPRY4 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 30
    • 13
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    600-401-697
    Hersteller
    Rockland
    Verwendungszweck
    Sprouty-4 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human Sprouty-4 protein.
    Produktmerkmale
    Synonyms: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
    Top Product
    Discover our top product SPRY4 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: 1 μg/mL to 10 μg/mL

    Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract.  Some non-specific banding may be observed using this antibody.

    Western Blot Dilution: 1:100 - 1:1,000

    ELISA Dilution: 1:3,000 - 1:12,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
    Andere Bezeichnung
    SPRY4 (SPRY4 Produkte)
    Hintergrund
    Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.  SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
    Gen-ID
    81848
    NCBI Accession
    NP_001120968
    UniProt
    Q9C004
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