SPRY4 Antikörper (AA 275-299)
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- Target Alle SPRY4 Antikörper anzeigen
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
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Bindungsspezifität
- AA 275-299
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser SPRY4 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Hersteller Produkt- Nr.
- 600-401-697
- Hersteller
- Rockland
- Verwendungszweck
- Sprouty-4 Antibody
- Kreuzreaktivität (Details)
- This affinity purified antibody is directed against human Sprouty-4 protein.
- Produktmerkmale
- Synonyms: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4
- Aufreinigung
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
Immunogen Type: Conjugated Peptide
- Isotyp
- IgG
- Top Product
- Discover our top product SPRY4 Primärantikörper
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- Applikationshinweise
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Immunohistochemistry Dilution: 1 μg/mL to 10 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
Western Blot Dilution: 1:100 - 1:1,000
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.1 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
- Andere Bezeichnung
- SPRY4 (SPRY4 Produkte)
- Hintergrund
- Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
- Gen-ID
- 81848
- NCBI Accession
- NP_001120968
- UniProt
- Q9C004
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