This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kDa in size corresponding to RAD54 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
RAD54, also known as hHR54, HR54, hRAD54 and RAD54A, belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA pairing, and stimulate DNA recombination. Synonyms: DNA repair and recombination protein RAD54 like antibody, hHR 54 antibody, hHR54 antibody, HR 54 antibody, HR54 antibody, hRAD 54 antibody, hRAD54 antibod