PNKP Antikörper (Internal Region)

Produktnummer ABIN129595

Hersteller Produkt- Nr.: 600-401-480

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PNKP in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und Schimpanse.

Kurzübersicht für PNKP Antikörper (Internal Region) (ABIN129595)

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reaktivität: Human, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNKP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-PNKP Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-480

Hersteller: Rockland

Verwendungszweck: PNK Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human PNK protein.

Produktmerkmale: Synonyms: rabbit anti-PNKP antibody, rabbit anti PNK antibody, rabbit anti-polynucleotide kinase antibody, Bifunctional polynucleotide phosphatase/kinase, Mammalian PNK, PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 57 kDa in size corresponding to PNK by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:300 - 1:1,500

ELISA Dilution: 1:3,000 - 1:12,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.46 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Andere Bezeichnung: PNKP

Hintergrund: Background: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage.  PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Gen-ID: 11284, 31543419

UniProt: Q96T60

Pathways: DNA Reparatur, Nucleotide Phosphorylation