POLK Antikörper (AA 817-830)

Produktnummer ABIN129559

Hersteller Produkt- Nr.: 600-101-295

Produktdetails anti-POLK Antikörper

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Bindungsspezifität: AA 817-830

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POLK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 600-101-295

Hersteller: Rockland

Verwendungszweck: POLK Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human POLK protein.

Produktmerkmale: Synonyms: goat Anti-POLK antibody, goat Anti-DNA polymerase kappa antibody, DINB homolog 1 (E. coli) antibody, DINB protein antibody, DINB1 antibody, DINP antibody, DNA damage inducible protein b antibody, POLQ antibody

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLK by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 89.0 and 76.4 kDa.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.4 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu POLK

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Andere Bezeichnung: POLK (POLK Produkte)

Hintergrund: Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair.  POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity.  POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

Gen-ID: 51426, 59798438

UniProt: Q9UBT6

Pathways: DNA Reparatur